Exosomal miR-182-5p from breast cancer cells reprogram tumor-associated macrophages and promote triple-negative breast cancer progression by targeting Notch1 in macrophages.

ABSTRACT:

Introduction

The regulatory role of miR-182 in breast cancer malignancy and macrophage reprogramming is well established. However, the mechanisms through which miR-182 overexpression in tumor cells influences macrophage polarization remain elusive.

Material and methods

After transfection with miR-182-5p mimics, inhibitors, and controls for 24 h, exosomes were extracted by differential centrifugation from transfected MDA-MB-231. Macrophages were co-cultured with these exosomes to illustrate the regulative effects of exo-miR-182-5p reprogram macrophage. Furthermore, breast cancer cells were co-cultured with exo-miR-182-5p reprogrammed M2 macrophages to demonstrate the effects of reprogrammed M2 macrophages to influence breast cancer progression. After all, these findings were

SUBMITTER: Li H 

PROVIDER: S-EPMC12509822 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature