Unsupervised multiscale clustering of single-cell transcriptomes to identify hierarchical structures of cell subtypes.

ABSTRACT:

Background

Cell clustering is an essential step in uncovering cellular architectures in single-cell RNA sequencing (scRNA-seq) data. However, the existing cell clustering approaches are not well designed to dissect complex structures of cellular landscapes at a finer resolution.

Results

Here, we develop a multiscale clustering (MSC) approach to construct a sparse cell-cell correlation network for unsupervised identification of de novo cell types and subtypes across multiple resolutions. Based upon simulated silver- and gold-standard data as well as real scRNA-seq data in diseases, MSC demonstrates significantly improved performance compared to established benchmark methods and reveals a biologically meaningful cell hierarchy to facilitate the discovery of novel disease-assoc

SUBMITTER: Song WM 

PROVIDER: S-EPMC12509883 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature