LDL transcytosis passes through the trans-Golgi network and requires Rab10.

ABSTRACT: Atherosclerosis begins with the subendothelial retention of LDLs from the circulation. While LDL transcytosis across the endothelium is mediated by scavenger receptor class B type I and activin-like kinase receptor 1 and is usually independent of LDL receptor, the intracellular mechanisms and route of LDL transcytosis remain unclear. Using total internal reflection fluorescence microscopy in LDL receptor-depleted human coronary artery endothelial cells, we found that LDL transcytosis can proceed both directly and indirectly from an intracellular compartment. During LDL transcytosis, LDL was observed to colocalize with the Golgi apparatus over time, specifically with the trans-Golgi network marker TGN46. Systematic examination of endothelial Rab proteins known to regulate Golgi traffic iden

SUBMITTER: Winnie Ho TW 

PROVIDER: S-EPMC12510203 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature