Glypican 1 mechanosensing mediates eNOS uncoupling during hydrostatic pulmonary edema.

ABSTRACT: Hydrostatic pulmonary edema is a life-threatening condition caused by an acute increase in pulmonary capillary pressure. The molecular mechanisms whereby hydrostatic pulmonary edema develops are unresolved. The pulmonary endothelial glycocalyx is a mechano-sensitive signaling layer known to regulate lung endothelial permeability. Within the glycocalyx, membrane-bound heparan sulfate proteoglycans (HSPGs) are putative mechano-sensors. Herein, we investigated if the membrane-bound HSPG glypican 1 is a mechanosensor in the lung vasculature and its role in hydrostatic pulmonary edema progression. Using an isolated perfused lung system, we showed that glypican 1 knockout mice (Gpc1^-/-) are protected from pressure-induced lung edema, a phenotype associated with impaired 70 KDa dextran

SUBMITTER: Thota LNR 

PROVIDER: S-EPMC12510217 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature