Biological and molecular characterisation of in vitro selected miltefosine-resistant Leishmania amazonensis lines.

ABSTRACT: Miltefosine (MTF) is currently the only available oral treatment for leishmaniasis. However, increasing reports of therapeutic failure have raised concerns about emerging resistance. This study aimed to investigate the effects of reduced MTF susceptibility loss in the protozoan parasite Leishmania (Leishmania) amazonensis, with a particular focus on its impact on key biological and molecular parameters. Two distinct Leishmania lines (LaR-40 Line 1 and Line 2) were generated through stepwise in vitro selection with increasing concentrations of MTF, reaching up to 40 μM MTF. They were compared to their wild-type counterpart (LaWT). After 12 weeks of selection, LaR-40 promastigotes exhibited IC₅₀ values that were 4- to 8-fold higher than those of LaWT, with resistance remaining sta

SUBMITTER: Neira LF 

PROVIDER: S-EPMC12510227 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature