PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder.

ABSTRACT: This paper presents the design, construction, operation, and evaluation of PowderBot, a purpose-built, open-source, low-cost machine (∼US$ 5000) that automates DNA extraction from ungerminated seeds. The device drills into the seed cotyledon, where the genetic information of the prospective plant is stored. It then transfers the pure, powdered samples directly to well-plates for analysis. This reduces time and other research resources and can accelerate crop varietal improvement, ultimately contributing to more efficient and successful crop breeding programs. At CIAT́s campus, we have validated the method for obtaining seed-tissue material from common beans for DNA extraction and subsequently genotyping agronomically interesting lines, using the bc-3 molecular marker. Three genotyping tria

SUBMITTER: Diaz H 

PROVIDER: S-EPMC12510228 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature