Bringing Attomolar Detection to the Point-of-Care with Nanopatterned DNA Origami Nanoantennas.

ABSTRACT: Creating increasingly sensitive and cost-effective nucleic acid detection methods is critical for enhancing point-of-care (POC) applications. This requires highly specific capture of biomarkers and efficient transduction of capture events. However, the signal from biomarkers present at extremely low amounts often falls below the detection limit of typical fluorescence-based methods, necessitating molecular amplification. Here, we present single-molecule detection of a non-amplified, 151-nucleotide sequence specific to antibiotics-resistant Klebsiella pneumoniae down to attomolar concentrations, using Trident NanoAntennas with Cleared HOtSpots (NACHOS). This NACHOS-diagnostics assay leverages a compact microscope with a large field-of-view, including microfluidic flow to enhance capturing e

SUBMITTER: Yaadav R 

PROVIDER: S-EPMC12510279 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature