Natural variation in the ZmPIMT1 promoter enhances seed aging tolerance by regulating PABP2 repair in maize.

ABSTRACT: PROTEIN L-ISOASPARTYL O-METHYLTRANSFERASE (PIMT) promotes seed vigor by repairing damaged proteins. However, whether PIMT variants have arisen during maize (Zea mays) domestication remains unknown. Here, we found 2 variants in the ZmPIMT1 promoter. The ZmPIMT1  Hap C7-2 promoter exhibited stronger activity than the ZmPIMT1  Hap Z58 promoter. Maize inbred lines carrying the ZmPIMT1  Hap C7-2 promoter had greater seed vigor than ZmPIMT1  Hap Z58 lines in a population of Zhengdan 958 recombinant inbred lines (RILs) and a maize inbred population. By characterizing the maize zmpimt1 knockdown mutant, ZmPIMT1-overexpressing maize and Arabidopsis thaliana heterologous ZmPIMT1 overexpression lines, we demonstrated that ZmPIMT1 positively regulates seed vigor. Co-IP and LC-MS/MS assays showed that

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12510314 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature