Discovery of microbial glycoside hydrolases <i>via</i> enrichment and metaproteomics.

ABSTRACT: The immense microbial diversity on Earth represents a vast genomic resource, yet discovering novel enzymes from complex environments remains challenging. Here, we combine a microbial enrichment with metagenomics and metaproteomics to facilitate the identification of microbial glycoside hydrolases that operate under defined conditions. We enriched microbial communities on the carbohydrate polymer pullulan at elevated temperatures under acidic conditions. Pullulan is a natural polysaccharide composed of maltotriose units linked by α-1,6-glycosidic bonds. Pullulan, along with its hydrolyzing enzymes, has broad applications across various industries. The enrichment inocula were sampled from thermophilic compost and from soil from the bank of a pond. In both cases, Alicyclobacillus was i

SUBMITTER: van Ede JM 

PROVIDER: S-EPMC12510369 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature