Hypoxia-Driven Histone Modifications Govern Gene Regulation for Mature Eye Lens Formation.

ABSTRACT:

Purpose

Evidence suggests that the hypoxic environment of the lens regulates the expression of genes required for lens formation and function. Here, we tested the hypothesis that hypoxia regulates these genes through the induction of specific histone modifications.

Methods

Global levels of hypoxia-induced histone modifications were determined in cultured day 13 chick lenses exposed to 1% oxygen. The genome-wide localizations of H3K27ac and H3K4me3 were identified by cleavage under target and release using nuclease analysis and mapped to within 5 kb of the transcriptional start sites of genes activated or repressed by hypoxia identified by RNA sequencing. The requirement for these histone modifications for hypoxia-induced gene expression was determined using inhibitors of his

SUBMITTER: Brennan L 

PROVIDER: S-EPMC12510386 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature