Isolation, identification and antibacterial activity of endophytes from the seeds of Panax japonicus.

ABSTRACT:

Methods

The streak plate method isolated the endophytic bacteria from the seeds. The isolated endophytes were identified by morphology, ITS and 16S rDNA sequence analysis. The antibacterial activity of the isolated endophytes and their metabolites was studied by disk method.

Results

The seed was disinfected with 75% alcohol for 30s and 1% HgCl2 for 9 min, and the contamination rate was the lowest (3.33%). Seven endophytic fungi strains were isolated from the seeds of P. japonicus, named PjZ1, PjZ2, PjZ3, PjZ4, PjZ5, PjZ6, and PjZ7, respectively, four strains belonged to the genus Fusarium tricinctum, one to Fusarium reticulatum, one to Fusarium sarcochroum, and one to Alternaria alternata; Three strains of endophytic bacteria were isolated and named PjX1, PjX2 and PjX3, resp

SUBMITTER: Jin R 

PROVIDER: S-EPMC12510572 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature