Role of the Multicopper Oxidase CueO in Copper Homeostasis Under Anaerobic Conditions in Enterobacteria.

ABSTRACT: The periplasmic multicopper oxidase CueO plays a crucial role in copper detoxification in enterobacteria. CueO contains a catalytic site, the Cu-T1 center, and a methionine-rich (Met-rich) domain capable of binding copper. This enzyme oxidizes cuprous ions (Cu⁺) to the less toxic cupric ions (Cu²⁺), coupled with oxygen reduction. This oxygen dependence has established CueO's role in alleviating copper stress under aerobic conditions, but its function in anaerobic environments remains uncertain. In this study, we demonstrated that under anaerobic conditions and copper stress in E. coli and S. Typhimurium, CueO is produced and contributes to copper homeostasis through its catalytic activity. Using CueO variants with either a mutated catalytic site or a deleted Met-rich

SUBMITTER: Becam J 

PROVIDER: S-EPMC12510620 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature