Automation in microinjection for zebrafish pericardial space with image-based motion control and batch agarose microplate.

ABSTRACT: Microinjection enables the precise delivery of substances into specific areas of small animals, such as zebrafish, whose xenograft models can be a promising platform for developing rapid and personalized cancer therapies. However, manual microinjection exhibits experimental variability and low reproducibility, as it relies on the expertise of researchers. To address these problems, automated microinjection systems have been developed in recent years. In this study, we propose a microrobotic system based on an image recognition AI model that extracts key feature points to define the pericardial space in zebrafish larvae at 2 days post-fertilization. Using the geometric relationships among feature points, the system optimizes the glass capillary insertion motion for precise microinjection. W

SUBMITTER: Lee HJ 

PROVIDER: S-EPMC12510664 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature