Development and validation of an analytical method for determination of bongkrekic acid in biofluids for toxin monitoring.

ABSTRACT: Bongkrekic acid (BKA) poisoning is a severe foodborne illness with a high mortality rate. This study aimed to identify BKA in postmortem tissues from poisoning victims in Taiwan and to develop a reliable analytical method for detecting BKA in biofluids to aid clinical diagnosis and treatment. BKA was identified in postmortem samples using high-resolution mass spectrometry (HRMS) and confirmed with an independent ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method. To meet clinical needs, a UHPLC-MS/MS method was developed and validated for BKA detection in plasma and urine. Method optimization included adjustments to ion source conditions for multiple reaction monitoring (MRM) transitions and avoidance of glass vials due to BKA adsorption

SUBMITTER: Shieh YT 

PROVIDER: S-EPMC12510690 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature