Uncovering hidden protein modifications with native top-down mass spectrometry.

ABSTRACT: Protein modifications drive dynamic cellular processes by modulating biomolecular interactions, yet capturing these modifications within their native structural context remains a significant challenge. Native top-down mass spectrometry promises to preserve the critical link between modifications and interactions. However, current methods often fail to detect uncharacterized or low-abundance modifications, limiting insights into proteoform diversity. To address this gap, we introduce precise and accurate Identification Of Native proteoforms (precisION), an interactive end-to-end software package that leverages a robust, data-driven fragment-level open search to detect, localize and quantify 'hidden' modifications within intact protein complexes. Applying precisION to four therapeutically re

SUBMITTER: Bennett JL 

PROVIDER: S-EPMC12510877 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature