Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against <i>Pseudomonas aeruginosa</i>.

ABSTRACT: The rising interest in essential oils (EOs) as antimicrobial agents demands evaluation frameworks that provide structured, reproducible assessments. In this study, we examined the strain-dependent response of Pseudomonas aeruginosa to pharmacopoeia-grade Thyme Essential Oil (obtained from Thymus vulgaris L., TEO) or polyhexamethylene biguanide antiseptic (PHMB) using a panel of ten genetically diversified strains in planktonic and biofilm forms, and by complementary in vitro models. Chemical composition of TEO was assessed using Gas Chromatography-Mass Spectrometry (GC-MS), and the main components were thymol, p-cymene, and γ-terpinene. Despite uniform test conditions, we observed striking inter-strain variability: TEO Minimal Inhibitory Concentrations (MICs) differed

SUBMITTER: Brozyna M 

PROVIDER: S-EPMC12510957 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature