In silico-derived <i>Actinobacillus equuli</i>-specific DNA markers and development of associated PCR assays.

ABSTRACT: The ability to accurately and rapidly identify causative agents of infectious diseases facilitates precise treatment, improves clinical outcomes, and augments epidemiology studies. For many veterinary and zoonotic pathogens, however, simple molecular tests for species identification are not available. Actinobacillus equuli causes severe diseases, such as sleepy foal disease, septicemia, and meningitis in horses and pigs. A. equuli can also cause severe diseases in humans bitten by infected animals. Existing A. equuli identification methods are biochemical tests, 16S rRNA gene amplification followed by DNA sequencing, and MALDI-TOF MS. Nonetheless, differentiating among Actinobacillus spp. by these methods is still challenging. We identified novel DNA markers spe

SUBMITTER: Songsungthong W 

PROVIDER: S-EPMC12511006 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature