Advancing hybrid modeling of Saccharomyces cerevisiae fermentation with mixed carbon sources and urea in a mini-stirred tank reactor.

ABSTRACT: Saccharomyces cerevisiae is indispensable to industrial fermentation; however, many existing models fail to adequately represent the metabolic complexity of its growth on mixed carbon sources in defined media. In this study, we introduce a novel hybrid modeling framework for the batch cultivation of S. cerevisiae, utilizing sucrose, glucose, and fructose as carbon sources, and urea as a nitrogen source. The model decisively captures critical phenomena under aerobic conditions, including the Crabtree effect, diauxic shifts, and sequential sugar utilization-critical areas frequently oversimplified in current models. By integrating mechanistic kinetics with data-driven enhancements, the hybrid model significantly improves predictive accuracy relative to the purely mechanistic baseline, reduci

SUBMITTER: Valencia-Velasquez J 

PROVIDER: S-EPMC12511178 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature