R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration.

ABSTRACT:

Background

In recent years, skeletal muscle has been recognized not only as a locomotor organ but also as a secretory organ. The bioactive molecules it releases, known as myokines, act on distant organs through the circulation and locally on muscle tissue. Previously, we identified R-spondin 3 (Rspo3) as a Type I fiber-specific myokine that promotes myoblast differentiation into Type I fibers in vitro. In this study, we further investigated whether Rspo3 is involved in regulating Type I fiber determination in vivo.

Methods and results

We generated tamoxifen-induced muscle-specific Rspo3-deficient mice and found that Rspo3 deficiency impaired Type I fiber determination during muscle regeneration. In contrast, transient overexpression of Rspo3 through in vivo electroporation e

SUBMITTER: Hu T 

PROVIDER: S-EPMC12511182 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature