Nano-Luciferase complementation assay of human herpesvirus 8 chemo/cytokine-receptor interactions.

ABSTRACT: Some viruses encode proteins that mimic host chemokines and/or cytokines to modulate the host immune responses against infection. Human herpesvirus 8 (HHV-8), also termed Kaposi sarcoma-associated herpesvirus (KSHV), encodes three viral CC-type chemokines (vCCLs 1-3) and an interleukin-6 ortholog (vIL-6). Therapeutic targeting of the vCCLs and vIL-6 has yet to be established despite their importance in immune evasion and transformation by HHV-8. The present study aimed to design a robust and straightforward method to assay receptor binding and downstream signaling using a Nano-Luciferase Binary Technology reporter system (NanoBiT); with this approach, we verified the direct interactions of vCCLs 1-3 with their established receptors. Furthermore, screening with a comprehensive set of human

SUBMITTER: Palande V 

PROVIDER: S-EPMC12511433 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature