Effects of luteal blood flow on endometrial progesterone concentrations and gene expression in Japanese Black cows.

ABSTRACT: Luteal blood flow (LBF) is essential for progesterone (P₄) biosynthesis in the corpus luteum (CL) and affects bovine fertility. However, the mechanism by which LBF affects fertility remains unclear. This study was conducted to investigate the effects of LBF on endometrial P₄ concentrations and gene expression. Endometrial biopsies and blood samples were collected from 13 Japanese Black cows after ultrasound examination on Day 7 (Day 0 = day of estrus). Based on LBF, the cows were divided into low- (LV; n = 5), medium- (MV; n = 2), and high-vascularity (HV; n = 6) groups. Plasma and endometrial P₄ concentrations were measured using enzyme immunoassays. RNA sequencing was performed to compare the endometrial gene expression profiles from three cows in each of

SUBMITTER: Kanazawa T 

PROVIDER: S-EPMC12511780 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature