Unveiling the Structural and Biochemical Characteristics of an Acidophilic β-Mannanase from Soybean (<i>Glycine max</i>).

ABSTRACT: Endo-1,4-β-mannanase (EC 3.2.1.78) cleaves β-1,4-mannans in cell walls, facilitating endosperm softening and seed germination. Here, we present the structural and biochemical characterization of GmMAN19-1, a GH5_7 β-mannanase from soybean (Glycine max), a crop with substantial agricultural importance. GmMAN19-1 is specifically expressed in cotyledons during postgermination and exhibits acidophilic activity optimal at pH 4.6 and 40 °C. Crystal structures of GmMAN19-1 were determined at 1.39 and 2.62 Å in the apo form and in complex with mannopentaose (M5), respectively. The structure adopts a canonical (α/β)₈ TIM barrel fold with a V-shaped active site groove. Notably, two distinct M5 binding modes were identified, suggesting dual functionality involving hydrolytic and tra

SUBMITTER: Lin CJ 

PROVIDER: S-EPMC12512182 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature