Single-cell RNA sequencing reveals that Danggui Buxue Tang decoction facilitates wound healing after anal fistula by promoting M2 macrophage polarization.

ABSTRACT:

Background

We aim to employ single-cell RNA (scRNA) sequencing technology to investigate potential regulatory mechanism of Danggui Buxue Tang (DBT) in wound healing for its utilization in post-anal fistula surgery recovery.

Methods

Fistula-like wound model in mice was established and administered DBT to assess its effects. Mice were divided into control and DBT groups and collected samples on the first day and 7th day after model establishment. The DBT was prepared from Astragalus membranaceus and Angelica sinensis. ScRNA sequencing was performed on each group.

Results

Our results showed that DBT treatment obviously reduced wound area in mice with anal fistula through activation of OPN/PI3K/Akt/eNOS signaling. Furthermore, the results of scRNA sequencing showed that a

SUBMITTER: Pang X 

PROVIDER: S-EPMC12512633 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature