Comparative genomic analysis of duck plague virus reveals evolutionary divergence between vaccine and contemporary field isolates in Thailand.

ABSTRACT: Duck Plague Virus (DPV), an alphaherpesvirus, causes significant morbidity and mortality in waterfowl and remains a persistent threat to duck farming across Asia. This study reports the first complete genomic characterization of DPV isolates from Thailand including a commercial vaccine strain (DPVac) and two contemporary field isolates (DPV7 and DPV8). Using Oxford Nanopore Technologies (ONT) and hybrid Illumina-ONT sequencing, we successfully assembled complete genomes ranging from 160,511 to 163,789 bp. The assembled genomes showed high sequence identity to the European reference strain DEV 2085. Comparative genomic analysis revealed structural differences in the UL and US regions. Phylogenetic reconstruction based on core genome single-nucleotide polymorphisms (SNPs) placed DPV7 within

SUBMITTER: Kruasuwan W 

PROVIDER: S-EPMC12512635 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature