Lipidomics changes in bronchoalveolar lavage fluid of refractory mycoplasma pneumoniae pneumonia: LC-MS-based analysis of potential biomarkers and pathogenesis.

ABSTRACT:

Background

Previous studies have reported the application of metabolomics in infectious diseases, but little is known about the potential function of bronchoalveolar lavage fluid (BALF) metabolites in children with (mycoplasma pneumoniae pneumonia) MPP and (refractory mycoplasma pneumoniae pneumonia) RMPP.

Methods

In this study, untargeted lipidomic analysis of BALF in MPP patients (n = 14) and RMPP patients (n = 14) was performed based on the liquid chromatograph-mass spectrometry (LC-MS) method. Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) was applied to analyze the resultant dataset. Differential metabolites with variable importance in the projection (VIP) >1.5, P < 0.05, and |log2FC| > 1 were subjected to multivariate r

SUBMITTER: Ma M 

PROVIDER: S-EPMC12512669 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature