Prospective multicenter validation of a next-generation sequencing panel using cytology specimens for lung cancer: cPANEL.

ABSTRACT:

Background

There are no prospective studies to estimate whether cytology specimens can replace tissue samples using lung cancer gene panel analysis. We evaluated the success rate of gene panel testing and nucleic acid yield and quality when using cytology specimens for lung cancer over tissue specimens.

Methods

In this prospective study, clinical cytology specimens collected via transbronchial brushing, needle aspiration washing, and pleural effusion were stored in a nucleic acid stabilizer. The primary endpoint was the superior success rate of gene analysis using cytology specimens over the conventional success rate using tissue specimens.

Results

The full analysis set included 248 cases. The success rate for gene panel analysis using cytology specimens was 98.4% (95

SUBMITTER: Morikawa K 

PROVIDER: S-EPMC12512683 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature