Annexin A2 facilitates porcine circovirus type 2 infection by mediating viral attachment to host cells and interacting with the capsid protein.

ABSTRACT: Porcine circovirus type 2 (PCV2) causes substantial economic losses globally. Although glycosaminoglycans (GAGs) have been identified as the general cell receptors responsible for PCV2 binding and subsequent cell infection, other critical protein(s) may also be involved. Our objective was to explore key host proteins that mediate PCV2 infection. The host protein annexin A2 (ANXA2) was observed to interact with PCV2 virus-like particles (VLPs) through immunoprecipitation (IP) and liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) experiments. Furthermore, knockdown of ANXA2 or the inhibitor A2ti-1 significantly reduced PCV2 replication in host cells. Notably, the addition of ANXA2-specific antibodies to cell culture or the preincubation of PCV2 with recombinant ANXA2 significantly re

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12512739 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature