Transcriptomic and metabolomic analyses reveal novel R2R3-MYB transcription factors control anthocyanin biosynthesis in broccoli.

ABSTRACT: Green broccoli is a very frequently consumed, nutrient-rich vegetable, whereas purple broccoli, known for its high anthocyanin content, is not so common. To investigate the anthocyanin biosynthesis in broccoli, one green broccoli and four purple broccolis were subjected to integrative analyses with metabolomics and transcriptomics. Our comprehensive examination revealed that in difference to other Brassica vegetables, the main anthocyanins accumulating in purple broccoli are delphinidin compounds, especially delphinidin-3-O-glucoside-5-O-galactoside. Transcriptomic analysis demonstrated significant variations in the expression levels of genes such as BoF3'H, BoDFR, and BoANS among the samples, suggesting the involvement of upstream transcription factors, regulating these genes. Furthermore

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC12512743 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature