Size-dependent immunomodulation by MSC secretome: soluble factors target innate pathways, components larger than 100 kDa regulate T cell proliferation.

ABSTRACT:

Background

Mesenchymal stromal cells exert their immunoregulatory effects through a complex secretome constituted of soluble factors and extracellular vesicles (EVs). While the immunomodulatory activity of the secretome has been demonstrated, the contribution of each fraction remains poorly defined. In particular, there is little knowledge about which bioactive molecules are responsible for the effect.

Methods

Human peripheral blood mononuclear cells (PBMCs) were treated with resiquimod in presence of clarified or concentrated secretome by tangential flow filtration (TFF), or fractions derived from ultracentrifugation. Supernatant was collected and used to treat THP-1 dual cells, a reporter cell line, to evaluate NF-κB and IRF pathway immunomodulation. T cell proliferation w

SUBMITTER: Wong C 

PROVIDER: S-EPMC12512777 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature