Spatiotemporal methylome remodeling during fiber differentiation in Gossypium hirsutum.

ABSTRACT: Whole-genome methylome analysis reveals DNA methylation dynamics during upland cotton (Gossypium hirsutum) fiber development. DNA methylation levels initially increased and then decreased from -3 days post-anthesis (DPA) to 5 DPA, with a significant rise during the rapid elongation stage (5 DPA to 14 DPA), particularly in CHH methylation levels. Integrated transcriptome analysis links elevated DNA methylation to reduced demethylase gene expression (DME, ROS1, DML3). DNA methylation remodeling modulates fiber development through coordinated regulation of lipid metabolism, DNA replication, phosphatidylinositol signaling system and hormonal signaling pathways. Several key transcription factors, such as TCP14, HD1, HOX3, and MYB25-like, showed a strong correlation with DMRs, suggesting their r

SUBMITTER: Yu Z 

PROVIDER: S-EPMC12512907 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature