Integrated fibre-specific methylome and proteome profiling of human skeletal muscle across males and females with fibre-type deconvolution.

ABSTRACT:

Background

Skeletal muscle is an important organ for health and movement, largely driven by specific muscle fibres. However, the comparison of fibre-type-specific DNA methylation and protein abundance from the same sample presents challenges. By combining previous methodological approaches we were able to directly compare the methylome and proteome in Type I and Type II human skeletal muscle fibres in males and females.

Methods

We assessed the methylome using the EPICv2 Infinium array and the proteome using liquid chromatography tandem mass spectrometry (LC-MS/MS) from Type I and Type II fibre pools from both males ( n=7 ) and females ( n=5 ).

Results

We identified 5,689 robust differentially methylated regions (Fisher P-value <0.001 ) and found strong relationships b

SUBMITTER: Palmer AS 

PROVIDER: S-EPMC12512927 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature