EDC4 enhances multi-drug chemosensitivity in pancreatic cancer via GR50-based profiling.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer, with drug resistance significantly impeding effective chemotherapy. The clinical importance of key genes in PDAC chemoresistance remains unclear.

Methods

Cytotoxicity assays were conducted on eight PDAC cell lines treated with gemcitabine, albumin paclitaxel, irinotecan, 5-FU, and cis-platinum. Based on GR50 values, cell lines were categorized as chemosensitive (CS) or chemoresistant (CR). RNA-seq data from the Cancer Cell Line Encyclopedia were analyzed for differential gene expression. Various statistical methods, including univariate Cox, LASSO, random forest, and multivariate Cox regression, were employed to construct a risk signature. This model's effectiveness was validated using time-depende

SUBMITTER: Qin C 

PROVIDER: S-EPMC12512939 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature