A new approach for establishing multilocus sequence typing scheme: Staphylococcus capitis as an example.

ABSTRACT: Reliable and efficient typing methods for Staphylococcus capitis, an opportunistic pathogen of growing clinical and epidemiological concern, are urgently needed to support microbial epidemiological studies. In the study, we present a novel workflow for developing a multilocus sequence typing (MLST) scheme tailored to S. capitis. Core genome analysis of 603 high-quality S. capitis genomes revealed 2,065 core genes. A hierarchical filtering strategy, including gene, fragment, and combination filters, was applied to select loci that balance discriminatory power and cluster specificity. The final MLST scheme comprised fragments from seven genes: mntC, phoA, atpB_2, hisS, rluB, carB, and clpP. Using this approach, we assigned 39 sequence types (STs) and defined five clonal complexes. The MLST t

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12513078 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature