Development of a novel transformation system using zhongshengmycin in <i>Aspergillus oryzae</i> and <i>Aspergillus niger</i>.

ABSTRACT: Filamentous fungi such as Aspergillus oryzae and Aspergillus niger serve as valuable microbial cell factories with diverse applications. However, genetic manipulation in these fungi is often hindered by the lack of efficient resistance-based transformation systems. To address this challenge, we developed a novel transformation system for both A. oryzae and A. niger based on zhongshengmycin, a cost-effective streptothricin-class antibiotic, used in combination with the resistance gene encoding nourseothricin acetyltransferase (NAT). Our tests revealed that sensitization with SDS and EDTA-Na₂ disrupts cell membrane integrity, thereby enhancing the susceptibility of Aspergillus to zhongshengmycin. Through an attB/attP-mediated integration system,

SUBMITTER: Bi F 

PROVIDER: S-EPMC12513303 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature