Stability, accuracy, and clinical performance of enzymatic total CO₂ measurement: Evaluation of the Snibe and Roche assays.

ABSTRACT:

Objectives

To evaluate the stability, accuracy, and clinical performance of the Snibe CO₂ assay compared with the Roche CO₂ reagent and to investigate factors affecting total CO₂ measurements in different clinical conditions.

Methods

Total CO₂ was measured in patient samples using enzymatic assays. Stability was assessed over time, and CO₂ loss was examined in different blood collection tubes. Method comparison was conducted between the Snibe and the Roche CO₂ assays in various diseases.

Results

The Snibe CO₂ assay showed excellent linearity (r = 0.9997) and maintained stability for 42 days without recalibration, with control material deviations within ±5% of the target. CO₂ loss was observed in uncapped non-anticoagulant tubes (24.0% decline over 48 hours), but was l

SUBMITTER: Wei S 

PROVIDER: S-EPMC12513603 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature