¹H, ¹⁵N and ¹³C backbone resonance assignments of flap endonuclease from Plasmodium falciparum.

ABSTRACT: Flap endonuclease (FEN) enzymes are a group of metallonucleases that have essential roles in DNA repair and the maintenance of genomic resilience. They catalyse hydrolytic cleavage of a phosphodiester bond to remove 5'-flaps present on double-stranded DNA molecules formed during DNA replication. FEN locates a target scissile bond through the structural recognition of a pronounced bend in the double-stranded DNA substrate along with the presence of both a 5'-flap and a 1-nucleotide 3'-flap. FEN enzymes share a common structural architecture and are functionally conserved across all living organisms. In this work, we report the ¹H, ¹⁵N and ¹³C backbone resonance assignments of residues 2-349 of FEN from Plasmodium falciparum (PfFEN349) in its substrate-free s

SUBMITTER: do Aido-Machado R 

PROVIDER: S-EPMC12513906 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature