Identification and characterization of two AA9 lytic polysaccharide monooxygenases isolated from the enzymatic arsenal in Phanerochaete chrysosporium.

ABSTRACT: White rot fungi including Phanerochaete chrysosporium are known for their ability to mineralize plant-derived materials, such as cellulose, hemicellulose, and lignin, into CO₂ and H₂O. This process is achieved through a diverse array of hydrolytic and oxidative enzymes. However, the mode of action and specific characteristics of lytic polysaccharide monooxygenases (LPMOs) from P. chrysosporium are not well understood. In this study, two auxiliary activity (AA) family 9 genes from P. chrysosporium, PchAA9C and PchAA9F, were heterologously expressed in Pichia pastoris and functionally characterized. The recombinant PchAA9C and PchAA9F exhibited optimal activity at 60 °C and pH 6.0, with their activity significantly enhanced by 0.5-3.0 mmol/L ascorbic acid (P < 0.05). Su

SUBMITTER: Xu X 

PROVIDER: S-EPMC12514121 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature