B cells induced regulatory T cells attenuated the classical M1 polarization of mouse bone marrow-derived macrophages.

ABSTRACT: Regulatory T (Treg) cells are effective immunomodulators of adaptive and innate immune responses. Our previous studies have demonstrated that B-cell-induced CD4⁺Foxp3^- regulatory T cells, referred to as Treg-of-B cells, exert suppressive capacity, by inhibiting CD4⁺CD25^- T-cell proliferation and inflammasome activation. In the present study, Treg-of-B cells downregulated proinflammatory M1-like markers and partially induced M2-associated genes in unpolarized bone marrow-derived macrophages (BMDMs), as evidenced by RNA expression of Nos2, Arg1, Retnla, Mrc1, and Egr2. Treg-of-B cells decreased the RNA levels of Nos2, Tnfa, Cd86, and Cxcl9, and reduced the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and nitrite in LPS/interfero

SUBMITTER: Huang YP 

PROVIDER: S-EPMC12514155 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature