Epigenetic landscape of hormone-independent sexual dimorphism and characterization of canine XIST.

ABSTRACT: Sexual dimorphism in dogs (Canis lupus familiaris) manifests through pronounced differences in morphology, physiology, and disease susceptibility. Despite early neutering, the persistence of sex-specific differences highlights the need to investigate factors beyond sex hormones that contribute to these characteristics. We collected whole blood tissue from spayed female (n = 4) and castrated male (n = 4) beagles and performed whole genome bisulfite sequencing (WGBS) and RNA seq. To investigate hormone-independent sex dimorphism of DNA methylation in neutered dogs, we investigated differentially methylated genes (DMGs) between sexes and candidate molecular pathways. Furthermore, we analyzed sex-related correlations between gene expression and methylation levels. Sex-related differentially me

SUBMITTER: Oh SJ 

PROVIDER: S-EPMC12514204 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature