An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.

ABSTRACT: Common wheat and durum wheat are widely cultivated cereal crops, both of which are infected by the obligate biotrophic pathogen Blumeria graminis f. sp. tritici (Bgt), causing powdery mildew. Recently, Pm68 gene was identified on the short arm of chromosome 2B in the Greek durum wheat line TRI 1796, conferring resistance to this disease. Here, we have cloned Pm68 from TRI 1796 using an integrated approach of genetic mapping, association analysis and PacBio sequencing. Transgenic assays demonstrate that Pm68 mediated resistance is controlled by a pair of genetically linked nucleotide-binding leucine-rich repeat (NLR)-encoding genes, Pm68-1 and Pm68-2. Transient expression assays in Nicotiana benthamiana leaves reveal that the activation of Pm68-1 is positively modulated by Pm68-2, likely th

SUBMITTER: He H 

PROVIDER: S-EPMC12514284 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature