Deficiency of nNOS in adult-born dentate granule cells causes epilepsy.

ABSTRACT:

Objective

This study aimed to elucidate the molecular role of neuronal nitric oxide synthase (nNOS, encoded by Nos1) in adult-born dentate granule cells (DGCs) during temporal lobe epilepsy (TLE).

Methods

We used GFP-expressing retrovirus (RV) to analyze morphological changes in DGCs. Nos1 knockout (Nos1^-/-) mice were generated to assess whether nNOS deficiency would induce mossy fiber sprouting (MFS), affect neurogenesis, and observe the morphological changes of DGCs. Nos1 conditional knockout (nNOS cKO, Nos1^loxp/loxp) mice were generated, and pAAV-Nestin-Cre virus was used to selectively delete Nos1 in adult-born DGCs, followed by electroencephalogram examination.

Results

We found seizures induced ectopic location, hilar basal dendrites, and de

SUBMITTER: Huang H 

PROVIDER: S-EPMC12514378 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature