Anastomotic leakage prevention using dry-preserved fibroblast cell sheets in esophageal reconstruction.

ABSTRACT:

Background

Anastomotic leakage is a common and serious complication of esophageal reconstruction, and new methods are required for its prevention in clinical settings. We herein developed dry-preserved fibroblast cell sheets (dry sheets), which are easy to use and promote wound healing. This study aimed to investigate the efficacy of transplanting allogeneic dry sheets in preventing anastomotic leakage in a rat esophageal reconstruction model.

Methods

Allogeneic dry sheets were prepared from the rat oral mucosa. A rat esophageal anastomosis model was created, and two dry sheets were applied to cover the anastomotic sites. Anastomotic leakage incidence, burst pressure, histological findings, and collagen contents were compared between the control and dry sheet groups postoper

SUBMITTER: Kurazumi H 

PROVIDER: S-EPMC12514519 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature