Proximity and metabolic activity proxies in the tumor microenvironment as predictors of survival in high grade serous ovarian cancer.

ABSTRACT: High grade serous ovarian cancer (HGSOC) is a lethal gynecological malignancy, often detected at the late stages of disease. There is a need to identify predictors of therapeutic response in the tumor microenvironment (TME). Here, we used spatial proteomics to characterize the TME of 51 patients with HGSOC and identify features that contribute to patient survival. Using a 48-plex immunofluorescent protein panel, we analyzed tumor and immune cell interactions. We identified that more CD66⁺ cells in a 50 μm radius of tumor cells and more cytotoxic CD8 T-Cells within a 10 μm radius of the tumor boundary were associated with improved overall survival. The proximity of Tregs to some metabolically active tumor cells was favourable, while their proximity to less metabolically active tu

SUBMITTER: Berrell N 

PROVIDER: S-EPMC12514571 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature