Nucleolar protein DCAF13 promotes non-small cell lung cancer cell proliferation via facilitating rDNA transcription and ribosome biogenesis.

ABSTRACT: The nucleolus is an important nonmembranous organelle within the nucleus and is responsible for ribosome biogenesis. This process is frequently upregulated in tumors to support elevated protein synthesis and sustain tumor growth. The transcription of ribosomal DNA (rDNA) into pre-45S rRNA, a rate-limiting step in ribosomal biogenesis, has been reported to be enhanced to drive the progression of non-small cell lung cancer (NSCLC). However, the mechanism that upregulating rDNA transcription in NSCLC remains unclear. Here, we show that rDNA transcript levels were increased in NSCLC tumor tissues. Reduction of rDNA transcription by CX-5461, an RNA polymerase I inhibitor, significantly inhibited NSCLC cell proliferation. We found that the expression level of DDB1- and CUL4-associated factor 13

SUBMITTER: Wang XM 

PROVIDER: S-EPMC12514575 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature