Assessment of Serum 3-Epi-25-Hydroxyvitamin D₃, 25-Hydroxyvitamin D₃ and 25-Hydroxyvitamin D₂ in the Korean Population With UPLC-MS/MS.

ABSTRACT:

Background

The liquid chromatography-tandem mass spectrometry method (LC-MS/MS) identified an epimeric form of 25-hydroxyvitamin D₃ (3-epi-25-OH-D₃) concentrations. For the first time, the 3-epi-25-OH-D₃ was included in the Korea National Health and Nutrition Examination Survey (KNHANES) IX (from 2022 to 2024). In this study, we evaluated the results of 3-epi-25-OH-D₃ in KNHANES IX to determine its clinical status in Korea.

Methods

We measured 3-epi-OH-D₃, 25-OH-D₃, and 25-OH-D₂ concentrations using an Ultra Performance (UP) LC-MS/MS, and assessed the differences in concentration ranges based on gender and age among participants in this national project.

Results

The concentration range of 3-e

SUBMITTER: Cho SE 

PROVIDER: S-EPMC12514969 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature