Defined and refined: development of a minimal medium for Clostridium pasteurianum.

ABSTRACT: Even though microorganisms can often grow in defined media, they are frequently cultured in rich media containing complex components like yeast extract. A drawback of using rich media is the effect of secondary substrates from complex components on the metabolism, which can change the anabolism and the formation of products, complicating the interpretation of experimental results. Rich media containing yeast extract is generally used to grow Clostridium pasteurianum. In this work, we describe the development of a minimal medium for C. pasteurianum combining rational media design, transfers in batch bottles and continuous bioreactors experiments. The media were designed based on literature, the elements needed in the metabolism, and a general chemical formula for the composition of biomass.

SUBMITTER: Nadal Alemany N 

PROVIDER: S-EPMC12515203 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature