Global but not myeloid lineage-directed Girk3 deletion increases bone mass in female mice.

ABSTRACT: Germline and osteoblast-directed deletion of G protein-gated inwardly rectifying K⁺ channel 3 (Girk3) was recently shown to increase bone mass after 18 wk of age in male mice. Here, we show that germline Girk3 deletion also increases trabecular and cortical bone mass and increases the mechanical strength of the femur in female mice after 18 wk of age. Unlike male mice, however, osteoblast-directed Girk3 deletion using 2.3 kb-Col1a1-Cre does not increase bone mass in adult female mice. To discover mechanisms underlying high bone mass in female Girk3^-/- mice, bulk RNA-sequencing was performed on 2-d-old calvarial bone, revealing lower expression of proinflammatory cytokines such as IL-1β and IL-6 in Girk3^-/- mice. Accordingly, cytokines and chemokines are lar

SUBMITTER: Weaver SR 

PROVIDER: S-EPMC12515477 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature