Tanyu Tongzhi Formula relieves the progression of atherosclerotic plaque through lipid regulation and anti-inflammatory effects.

ABSTRACT:

Objective

Tanyu Tongzhi Formula (TTF), a clinically proven empirical prescription, has been utilized to treat atherosclerosis (AS) for decades. This study aimed to investigate the therapeutic mechanisms of TTF against AS by integrating bioinformatics, multi-omics, and experimental validation.

Methods

The metabolites of TTF in serum were identified using Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). Bioinformatics was employed to predict drug targets and mechanisms of action. ApoE^-/- C57BL/6J mice were fed a 12-week high-fat diet to establish an AS model and were treated with TTF crude extract (2.25 g/kg/d) via gavage. Interleukin 6 (IL-6) and interleukin 1β (IL-1β) were measured at weeks 6, 10, and 12. At the 12-week endpoint, aortic plaque

SUBMITTER: Chen T 

PROVIDER: S-EPMC12515906 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature