Single-cell proteomics characterization of bone marrow hematopoiesis with distinct Ras pathway lesions.

ABSTRACT:

Abstract

Aberrantly elevated Ras signals, triggered by various distinct genetic mutations, are frequent features in myeloid leukemias. Normal hematopoiesis requires perpetual and balanced production of different blood cell lineages by multipotent hematopoietic stem cells (HSCs). Stem and progenitor cells combine dormancy with proliferative drive and require finely tuned metabolism and protein translation rates. Because of the scarcity of stem cells, it has remained largely unknown how aberrantly elevated Ras signals may impact frequency, lineage potential, and quiescent metabolism in rare HSCs. Using single-cell proteomics and computational analyses, we characterized the effects of induced oncogenic mutant KRasG12D or overexpression of the Ras activator RasGRP1, compared with norma

SUBMITTER: Karra L 

PROVIDER: S-EPMC12516052 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature