A Novel C19orf47-AKT2 Chimeric RNA Generated by Cis-Splicing of Adjacent Genes Is Associated With Glioblastoma Prognosis.

ABSTRACT:

Objective

Malignant gliomas pose significant therapeutic challenges. This study aimed to identify and characterize a novel chimeric RNA in glioma and assess its clinical and functional significance for precision treatment.

Methods

The C19orf47-AKT2 chimeric RNAs were identified through RNA sequencing and validated by polymerase chain reaction. Their expression in tumor core and peritumoral tissues was quantified and compared via quantitative polymerase chain reaction, whereas their prognostic significance in glioblastoma was assessed using Kaplan-Meier analysis. The formation mechanism was investigated through genomic analysis, and western blotting was performed to assess fusion protein translation. The CCK-8 assay was performed to assess the chimeras' effect on glioma proli

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12516222 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature